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Clinical evaluation of recombinant human growth hormone injection in children with growth hormone deficiency

Ling HOU, Xiaoping LUO, Minlian DU, Huamei MA, Chunxiu GONG, Yuchuan LI, Shuixian SHEN, Zhuhui ZHAO, Li LIANG, Guanping DONG, Chaoying YAN, Hongwei DU

Frontiers of Medicine 2009, Volume 3, Issue 2,   Pages 171-176 doi: 10.1007/s11684-009-0027-4

Abstract: Recombinant human growth hormone (rhGH) has been widely used in the clinical treatment of growth hormoneof the current study was to evaluate the efficacy and safety of rhGH injection for the treatment of growthhormone deficiency (GHD) in children in China.insulin-like growth factor I (IGF-I), insulin-like growth factor binding protein 3 (IGFBP-3), and boneWe conclude that rhGH injection is a safe and effective drug for treatment of growth hormone deficiency

Keywords： recombinant human growth hormone     injection     growth hormone deficiency    

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Sensitivity of supplementation of thyroid hormone on treatment of idiopathic short-stature children duringtherapy with recombinant human growth hormone

Wei Wang, Shuqin Jiang, Zhirui Cui, Xiangyang Luo, Lingli Shi, Heli Zheng

Frontiers of Medicine 2018, Volume 12, Issue 5,   Pages 580-585 doi: 10.1007/s11684-017-0585-9

Abstract:

This study aimed to evaluate the effects of thyroid hormone supplementation on growth rate of childrenwith idiopathic short stature (ISS) and low-normal serum free thyroxine FT4 who were receiving growthhormone therapy.In children with ISS, the negative effect of thyroid hormone deficiency on growth rate should be consideredwhen FT4 level lies in the low-normal range prior to recombinant human growth hormone treatment.

Keywords： therapeutic     idiopathic short-stature children     free T4     the first year     recombinant human growth hormone    

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Overcoming resistance to endocrine therapy in hormone receptor-positive human epidermal growth factor

Wenjie Zhu, Binghe Xu

Frontiers of Medicine 2021, Volume 15, Issue 2,   Pages 208-220 doi: 10.1007/s11684-020-0795-4

Abstract: New targeted therapies have been developed to overcome resistance to endocrine therapy (ET) and improve the outcome of HR /HER2 advanced breast cancer (ABC). We conducted a meta-analysis and systemic review on randomized controlled trials evaluating various targeted therapies in combination with ET in HR /HER2 ABC. PUBMED and EMBASE databases were searched for eligible trials. Hazard ratios (HRs) for progression-free survival (PFS), odds ratios (ORs) for objective response rate (ORR), clinical benefit rate (CBR), and toxicity were meta-analyzed. Twenty-six studies with data on 10 347 patients were included and pooled. The addition of cyclin-dependent kinase 4/6 inhibitors to ET significantly improved median PFS (pooled HR= 0.547, <0.001), overall survival (pooled HR= 0.755, <0.001), and tumor response rates (ORR, pooled OR= 1.478, <0.001; CBR, pooled OR= 1.201, <0.001) with manageable toxicities (pooled OR= 3.280, <0.001). The mammalian targets of rapamycin inhibitors and exemestane were not clinically beneficial for this pooled population including ET-naïve and ET-resistant patients. Moderate improvement in PFS (pooled HR= 0.686, <0.001) yet pronounced toxicities (pooled OR= 2.154, <0.001) were noted in the combination of phosphatidylinositol-4,5-bisphosphate 3-kinase inhibitors with fulvestrant. Future studies are warranted to optimize the population and the dosing sequence of these available options.

Keywords： endocrine-resistant     HR+/HER2- advanced breast cancer     randomized clinical trials     meta-analysis     targeted therapy    

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High-expression of recombinant human concensus interferon-α by Pichia pastoris

HAO Yuyou, SHI Qiqi, HE Yun, ZHUANG Yingping, WANG Yonghong, ZHANG Siliang, CHU Ju, LIU Zhimin

Frontiers of Chemical Science and Engineering 2007, Volume 1, Issue 4,   Pages 399-403 doi: 10.1007/s11705-007-0073-x

Abstract: The present work focused on the high expression of recombinant human consensus interferon- (cIFN) by

Keywords： bioactivity     pastoris     maximum     induction     optimized    

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Construction of a universal recombinant expression vector that regulates the expression of human lysozyme

Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI

Frontiers of Agricultural Science and Engineering 2018, Volume 5, Issue 3,   Pages 382-389 doi: 10.15302/J-FASE-2018211

Abstract:

The mammary gland provides a novel method for producing recombinant proteins in milk of transgenic>) was modified twice by homologous recombination to produce a universal expression vector, and the humanpronuclei of fertilized mouse embryos, to successfully generate two transgenic mouse lines that expressed recombinanthuman lysozyme (rhLZ) in milk.

Keywords： BAC recombinant methods     gene expression     human lysozyme     transgenic mice     milk expression vector    

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Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice

Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 3,   Pages 179-184 doi: 10.15302/J-FASE-2014020

Abstract: Due to the limited yield of human BCHE (hBCHE) when purifying from human plasma, it is necessary to findIn this study, we cloned the gene into a human lactoferrin (hLF) bacterial artificial chromosome (BACExpression analysis showed that recombinant hBCHE (rhBCHE) was expressed efficiently in the mammary gland

Keywords： recombinant human butyrylcholinesterase (rhBCHE)     human lactoferrin bacterial artificial chromosome (hLF    

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Study of recombinant human IFN-α-2b bacilli Calmette–Guerin activated killer cells and against bladder

FAN Xiaodong, HAN Ruifa

Frontiers of Medicine 2007, Volume 1, Issue 4,   Pages 377-380 doi: 10.1007/s11684-007-0073-8

Abstract: Therefore, current studies aim at developing recombinant BCG (rBCG) strains secreting Th1-like cytokinesRecombinant IFN--2b-BCG, wild-type BCG (wBCG), wild-type BCG and IFN--2b were coincubated with PBMCsThe result of MTT showed that the proliferation of PBMC in the recombinant BCG group was more powerfulThe result of LDH release assay showed that the antitumor activity of BAK cells stimulated by RecombinantWe conclude that the recombinant BCG can activate more PBMCs to anti-bladder cancer than wild-type BCG

Keywords： Urology     powerful     2b-BCG     Tianjin institute     wild-type    

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Regulation of exogenous bFGF gene mediated by recombinant adeno-associated virus

Ke SONG, Nianjing RAO, Meiling CHEN, Yingguang CAO

Frontiers of Medicine 2009, Volume 3, Issue 2,   Pages 158-163 doi: 10.1007/s11684-009-0042-5

Abstract: The regulatory effect of basic fibroblast growth factor (bFGF) mediated by recombinant adeno-associatedRecombinant plasmid pAAV-S3-bFGF, and pSVneo were co-transfected into BHK-21 cells, then the recombinantThe titer of the recombinant rAAV2-tet-off-bFGF was determined by dot-blot assay.It was concluded that rAAV2-tet-off-bFGF could infect MC3T3 cells efficiently, and this recombinant system

Keywords： tetracycline regulatory system     adeno-associated virus     basic fibroblast growth factor     gene regulation    

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Atypical pituitary hormone–target tissue axis

Frontiers of Medicine 2023, Volume 17, Issue 1,   Pages 1-17 doi: 10.1007/s11684-022-0973-7

Abstract: However, a number of studies have shown that multiple types of pituitary hormone receptors are widelyEach pituitary gland-derived hormone exhibits a wide range of nonconventional biological effects in thesehormone, luteinizing hormone, adrenocorticotrophic hormone, and prolactin when they act on non-classicalorgans were summarized, defined by the novel concept of an “atypical pituitary hormone–target tissuethe coordinated regulation of environmental factors, genetic factors, and neuroendocrine hormones on human

Keywords： thyroid-stimulating hormone     follicle-stimulating hormone     luteinizing hormone     adrenocorticotrophichormone     prolactin    

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Molecular characterization of two suppressor of cytokine signaling 1 genes (

Xue XU,Jiannan ZHANG,Juan LI,Yajun WANG

Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 1,   Pages 73-83 doi: 10.15302/J-FASE-2015044

Abstract: predicted to encode a 207-amino acid protein, which shares high amino acid sequence identity (64%–67%) with humanpredicted to encode a 212-amino acid protein, which shares only 30%–32% amino acid sequence identity with humansystem, we further demonstrated that transient expression of and can significantly inhibit chicken growthhormone (GH)- or prolactin (PRL)-induced luciferase activities of Hep G2 cells expressing cGH receptortogether, these data suggest that both cSOCS1a and cSOCS1b may function as negative regulators of cytokine/hormone

Keywords： chicken     SOCS1a     SOCS1b     growth hormone     prolactin    

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Prokaryotic expression and bioactivity analysis of growth hormone and insulin-like growth factor-I from

Xu Yongjiang,Zang Kun,Liu Xuezhou,Shi Bao and Chen Shengyi

Strategic Study of CAE 2015, Volume 17, Issue 1,   Pages 67-73

Abstract:

The mature peptide domain of insulin-like growth factor-I from Platichthys stellatus were amplifiedwas subcloned into the prokaryotic expression vector pET-28a to successfully construct IGF-I/pET-28a recombinantof human embryonic kidney cells HEK293T at 0.6 μg/mL and inhibit the proliferation with 1.8 μgThe present results would be helpful for better understanding the roles of IGF-I in growth regulationand development of high effective additive for growth promotion of Platichthys stellatus.

Keywords： Platichthys stellatus； insulin-like growth factor-I； prokaryotic expression； bioactivity    

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How to judge the association of postmenopausal hormone therapy and the risk of breast cancer

Ling XU

Frontiers of Medicine 2010, Volume 4, Issue 3,   Pages 290-293 doi: 10.1007/s11684-010-0093-7

Abstract: The relevance of postmenopausal hormone therapy (HT) for breast cancer risk has been long debated, although

Keywords： breast cancer     postmenopausal hormone therapy     unopposed estrogen therapy     combined estrogen-progestin therapy    

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Expression and function of DMT1 without IRE in C6 cells mediated by recombinant adenovirus

Xixun DU*, Huamin XU*, Hong JIANG, Jun WANG, Lei WANG, Junxia XIE

Frontiers of Medicine 2009, Volume 3, Issue 1,   Pages 67-71 doi: 10.1007/s11684-009-0010-0

Abstract: In the present study, we constructed a recombinant adenovirus containing the gene of DMT1 without theI-digested pAdEasy1-DMT1-IRE was then transfected into E1-transformed human embryonic kidney cells (HEK293cells) , in which recombinant adenoviruses were generated within 7 to 10 days.PCR confirmed pAdEasy1-DMT1-IRE contained gene DMT1-IRE, indicating the successful construction of recombinant

Keywords： divalent metal transporter 1     recombinant adenovirus     homologous recombination     iron    

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Pituitary-ovarian hormone and follicular development of various combined oral contraceptives regimens

Huang Pei,Huang Xunbin

Strategic Study of CAE 2015, Volume 17, Issue 6,   Pages 16-20

Abstract: of estrogen and progestin, secondarily leading to suppression of endogenous gonadotropin and gonadal hormoneDuring the hormone-free interval (HFI), COCs users experience activation of their pituitary–ovarian

Keywords： COCs     HFI     hormone     follicle    

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for lung repair and regeneration in humans: key stem cells and therapeutic functions of fibroblast growth

Xuran Chu, Chengshui Chen, Chaolei Chen, Jin-San Zhang, Saverio Bellusci, Xiaokun Li

Frontiers of Medicine 2020, Volume 14, Issue 3,   Pages 262-272 doi: 10.1007/s11684-019-0717-5

Abstract: The recognition that human lungs can both repair and regenerate is quite novel, the concept has not beenWe present evidence that the human adult lung does repair and regenerate and introduce different wayscells, such as basal cells, broncho-alveolar stem cells, and alveolar type 2 cells, rely on fibroblast growth

Keywords： FGF     human lung     repair     regeneration     stem cells    

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Title Author Date Type Operation

Clinical evaluation of recombinant human growth hormone injection in children with growth hormone deficiency

Ling HOU, Xiaoping LUO, Minlian DU, Huamei MA, Chunxiu GONG, Yuchuan LI, Shuixian SHEN, Zhuhui ZHAO, Li LIANG, Guanping DONG, Chaoying YAN, Hongwei DU

Journal Article

Sensitivity of supplementation of thyroid hormone on treatment of idiopathic short-stature children duringtherapy with recombinant human growth hormone

Wei Wang, Shuqin Jiang, Zhirui Cui, Xiangyang Luo, Lingli Shi, Heli Zheng

Journal Article

Overcoming resistance to endocrine therapy in hormone receptor-positive human epidermal growth factor

Wenjie Zhu, Binghe Xu

Journal Article

High-expression of recombinant human concensus interferon-α by Pichia pastoris

HAO Yuyou, SHI Qiqi, HE Yun, ZHUANG Yingping, WANG Yonghong, ZHANG Siliang, CHU Ju, LIU Zhimin

Journal Article

Construction of a universal recombinant expression vector that regulates the expression of human lysozyme

Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI

Journal Article

Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice

Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI

Journal Article

Study of recombinant human IFN-α-2b bacilli Calmette–Guerin activated killer cells and against bladder

FAN Xiaodong, HAN Ruifa

Journal Article

Regulation of exogenous bFGF gene mediated by recombinant adeno-associated virus

Ke SONG, Nianjing RAO, Meiling CHEN, Yingguang CAO

Journal Article

Atypical pituitary hormone–target tissue axis

Journal Article

Molecular characterization of two suppressor of cytokine signaling 1 genes (

Xue XU,Jiannan ZHANG,Juan LI,Yajun WANG

Journal Article

Prokaryotic expression and bioactivity analysis of growth hormone and insulin-like growth factor-I from

Xu Yongjiang,Zang Kun,Liu Xuezhou,Shi Bao and Chen Shengyi

Journal Article

How to judge the association of postmenopausal hormone therapy and the risk of breast cancer

Ling XU

Journal Article

Expression and function of DMT1 without IRE in C6 cells mediated by recombinant adenovirus

Xixun DU*, Huamin XU*, Hong JIANG, Jun WANG, Lei WANG, Junxia XIE

Journal Article

Pituitary-ovarian hormone and follicular development of various combined oral contraceptives regimens

Huang Pei,Huang Xunbin

Journal Article

for lung repair and regeneration in humans: key stem cells and therapeutic functions of fibroblast growth

Xuran Chu, Chengshui Chen, Chaolei Chen, Jin-San Zhang, Saverio Bellusci, Xiaokun Li

Journal Article